1.
Effect Of Zinc Oxide Nanoparticles On Serum Lipid Profile And Liver Function Test In Alloxan-Induced Diabetic Rats
by Sidra Batool (2013-VA-592) | Dr. Khalid Abdul Majeed | Dr. Imtiaz Rabbani | Dr. Hafsa Zaneb .
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Diabetes mellitus is most common disorder affecting almost 25% of the world's population. Many researchers reported the role of metals in glucose metabolism and their defficiency cause the diabetes. Zn is found in all cells of body and play role to maintain blood sugar level and in the synthesis, storage, and secretion of insulin. Zn supplementation shows protective effect on total lipid, cholesterol, HDL and atherogenic index. Nanotechnology affects on the all parts of human life, animal, environment, and industrial life. In the present study I evaluate the anti-diabetic activity of ZnONPs and also the dose dependent anti-diabetic effects of medicine in alloxan induced diabetic rats.
Twenty five adult male rats were divided into five groups; Negative control, Positive control, and 3 groups for different treatment dose of ZnONPs (15 mg/kg BW), ZnONPs (25mg/kg BW) and ZnONPs (50 mg/kg BW). Diabetes induction had done in four groups, other than negative control, by subcutaneous administration of alloxan (120 mg/kg BW). Treatment was given to groups for 21 days. BW was recorded weekly. Blood samples were collected from animals of each group on 21 days after induction of diabetes by cardiac puncture to measure serum glucose level, serum lipid profile and liver function test. Data was analyzed by using SPSS software. Data was analyzed by using one-way ANOVA. The group differences were compared by the Duncan’s Multiple Range Test. Differences was considered significant at P < 0.05.
The obtained results showed that ZnONPs has efficiency to control the diabetes mellitus by reducing blood glucose levels as well as the increasing dose decreased the serum glucose level. Only ZnONPs 50mg/kg BW dose is efficient to reduce the muscle wastage due to diabetes in alloxan induce diabetic rats. This dose also works to maintain the serum ALT, TG, HDL-C, LDL-C levels. But this dose is not effective for body weight, AST, ALP, VLDL-C, cholesterol and AI.
The data obtained from this study also showed the dose dependent anti-diabetic activity of medicine as the dose of 50mg/kg BW is more effective to control the diabetes as compared to other two doses; 15mg/kg BW and 25mg/kg BW.
Availability: Items available for loan: UVAS Library [Call number: 2402-T] (1).
2.
In Vitro Comparison of Buffer Temperature on Electrophysiological & Histological Indices of Ruminal Epithelium
by Muhammad Jawad Masood Khan (2014-VA-817) | Dr. Imtiaz Rabbani | Dr. Khalid Abdul Majeed | Dr. Saima Masood.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: CD corrupted. Availability: Items available for loan: UVAS Library [Call number: 2698-T] (1).
3.
Effects Of Dietary Supplementation Of Drumstick Tree Leaf Powder On Anthropometric Parameters, Serum Lipid Profile And Thyroid Function Hormones In Rats Exposed To Thermal Stress
by Raja Majid Masoud (2015-VA-433) | Dr. Khalid Abdul Majeed | Dr. Imtiaz Rabbani | Dr. Saima Masood.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Introduction: There are a number of stressors which adversely affects the normal homeostasis
of the body. One of them is thermal stress which has marked damaging effect on the normal
physiology of an individual. Drumstick tree is a versatile plant from Moringaceae family.
Drumstick tree is a best food plant but it also has a large potential as a medicinal plant . Due to
rich source of flavonoids, β-carotenes, vitamin A & D, DST is known as a best natural antioxidant.
The leaves of Drumstick tree contain phenolic compounds, which have
hypocholesterolemic and hypolipodemic properties. It also possess cardio protective, hepatoprotective
and renal protective activities due to its rich anti-oxidant potential.
Hypothesis: It was hypothesized that Drumstick tree leaf powder supplementation will
ameliorate the growth performance, serum lipid profile and thyroid function in rats exposed to
thermal stress
Methodology: Adult female Wistar rats (n= 24) were procured and housed in stainless steel
cages in the experimental animal shed of department of Physiology, University of Veterinary and
Animal Sciences, Lahore-Pakistan. After acclimatization, rats were divided in to four groups
(One control and 3 DSTLP treated groups). Rats were exposed to high environmental
temperature(37±1°C) for one hour daily(12.00 Pm-1.00 Pm) throughout the experimental period
of 21 days for the induction of heat stress(Sinha. 2007). Anti-stress properties of Drumstick tree
leaf powder were examined by measuring biochemical parameters, i.e. anthropometry, serum
lipid profile, thyroid function hormones, serum cortisol, total protein, albumin and globulin
(Ohkawa et al. 1979). Analysis of all these parameters was carried out by using commercially
available kits provided by Human Diagnostics, Germany.
Summary
37
Results: Results from the present study revealed that Drumstick tree leaf powder
supplementation during thermal stress has a significant effect (P<0.05) on serum lipid profile,
total protein, albumin globulin, thyroid function hormones (T3 & T4) and cortisol. On the other
hand, Drumstick tree leaf powder demonstrated a non-significant effect (P>0.05) on
anthropometric parameters.
Statistical analysis: The data was analyzed by using one way analysis of variance (ANOVA)
using SPSS software. The group differences were compared by the Tukey’s Test. Differences
was considered significant at P < 0.05.
Conclusion: From this study, it was concluded that Drumstick tree leaf powder (MOLP) has a
potent antioxidant potential by lowering cholesterol, TG and LDL-cholesterol with marked
ameliorative effect on TP, albumin and globulin in heat stressed rats. Further studies are required
to explore the potential of other portions of this plant in heat stress. Availability: Items available for loan: UVAS Library [Call number: 2799-T] (1).
4.
Effect Of Aqeous Extract Of Catharanthus Roseuson The Electrophysiology Of Jejunum Infected With Escherichia Coli In Buffalo
by Qurrat ul Ain (2014-VA-530) | Dr. Imtiaz Rabbani | Dr. Khalid Abdul Majeed | Dr. Muhammad Nasir.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Animal products such as meat and milk are the primary source of proteins and amino acid that can be produced throughout the year. Diseases in animals initiate the biophysical and socio economic impact that varies from localized to global. Different pathogens including virus, bacteria and fungi cause different diseases in ruminants. Escherichia coli are one of the inhabitants of intestinal tract and are responsible for watery diarrhea, bloody diarrhea and hemorrhagic colitis. Diarrheal disease gives rise to global health problem and now a day many plants are used to treat the disease condition. Catharanthus roseusis one of the important medicinal plants used for the treatment of many diseases such as diarrheal, diabetics, wound healing and Alzheimer’s disease. C. roseus maintain the optimum performance of gut barrier by reducing the loss of minerals. The changes in the electrophysiology of jejunum with the addition of C.rouses were measured by Ussing chamber. Objective of this study was to investigate the effects of C.roseus on the barrier function of intestine influenced by E. coli.
Catharanthus roseus may reduce the detrimental effects induced by E.coli and improves the gut barrier function.
Five buffalos of uniform age and weight were selected and slaughter at local slaughter house. Jejunum was taken from the intestinal part and rinsed with standard buffer. Serosal layer was removed and tissue was mounted in using chamber. After incubation period of 15-20 mins different groups were treated with different treatments. Control with no treatment, group B was treated with 10% toxin of E.coli, group C was treated with 1.5% extract of C.rouses, group D was treated with 10% toxin of E.coli and 1.5% extract of C.rouses. After treatment the electrophysiological parameters like Isc and Gt was observed.
C.rouses affected the electrophysiology of isolated jejunum of buffalos by decreasing the short circuit current which was induced by the E.coli toxin. The decrease of current between the groups is significant (p value < 0.05). ΔIscwas also significant between the groups containing E. coli toxin and C. roseus containing solution. Conductance increased significantly after the addition of E. coli and C. roseus (p value < 0.05).
Data were analyzed by using completely randomized ANOVA followed by post-hoc Duncan’s multiple range tests. Level of significance was set at 0.05.
Catharanthus roseus treatment affects the electrophysiological indices of isolated jejunum epithelium of buffalos presumably by closing the chloride channels and enhances the ions absorption across the jejunum epithelium. This may leads to efficient mineral utilization and better health of animals.
Availability: Items available for loan: UVAS Library [Call number: 2836-T] (1).
5.
Effect Of Psyllium Seed Husk On Anthropometric Parameters, Lipid And Electrolyte Profile In Hypercholesterolemia Induced Rats
by Arfa Ibtisham (2015-VA-24) | Dr. Imtiaz Rabbani | Dr. Khalid Abdul Majeed | Dr. Sanaullah Iqbal.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Hypercholesterolemia is characterized by higher level of bad cholesterol in blood which shoots up the risk factor for cardiovascular diseases, hyperlipidemia, oxidative stress, weight gain and other metabolic disorders. Modern way of life is the reason to elevate these metabolic syndromes that lead to increased mortality rate. Psyllium husk is helpful in improving anthropometric parameters, high lipid profile, glucose level, appetite, oxidative status, hypertension and maintains liver and renal physiology. The effective use of this drug was for weight loss to control obesity.
The consumption of psyllium husk may lead to hypocholesterolemia, reduced body weight, oxidative stress and biological profile in rats. Twenty four male Wistar rats were taken and divided into four groups. Group-I (negative control) fed chow diet Group-II (positive control) was given chow diet mixed with 10g cholesterol for 42 days. Group-III (treatment group) hypercholesterolemic rats were fed with 5g psyllium husk for 21 days. Group-IV (treatment group) hypercholesterolemic rats were fed with 10g psyllium husk for 21 days. Blood samples were collected at the end of six weeks of study. Anthropometric parameters, organs weight, Lipid profile (TC, TC, LDL, HDL, Atherogenic index), glucose, oxidant and antioxidant levels, electrolytes, hepatic (AST, ALT) and renal (blood urea nitrogen, urea) functions (in blood liver and kidney) were studied. Data were analyzed using one way ANOVA on SPSS. Differences between the groups were compared by Duncan’s Multiple Range Test. Differences were considered significant at P < 0.05.
Anthropometric measurements, organs weight, lipid profile, glucose,urea and blood urea nitrogen showed significantly results, while,TG, atherogenic index ,electrolytes, oxidant and anti-oxidant ALT and AST showed no significantly values.
Availability: Items available for loan: UVAS Library [Call number: 2867-T] (1).
